By Harry ed. Sobotka
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Additional resources for Advances in Clinical Chemistry, Vol. 4
1 paper strips, 1 inch wide, cut to a satisfactory length for the tank to be used, are satisfactory. Standards are run separately from the urine but under the same conditions; and once the sugar has been tentatively identified, the urine is run again adding a known standard to the area of application of the urine which corresponds to the sugar suspected; if the standard and the “unknown” sugar are in fact identical, only one spot is produced on the chromatogram. Some investigators prefer a wider sheet of filter paper which permits several samples and standards to be run, side by side, on the same paper, In order to determine the Rf of a sugar, the chromatogram must be removed from the tank before the solvent has reached the end of the 40 JAMES B.
The difficulty in the chromatography of urinary sugars lies in the fact that glucose and galactose are not satisfactorily separable. We have found ethyl acetate:pyridine:water ( 4 : 10:3) very satisfactory for the detection and identification of the sugars usually found in urine, and glucose and galactose are separated. This system has the added advantage that it is not very sensitive to salts, whereas the systems described by Partridge ( P l ) required that the urine be deionized before application on paper.
Fructose is not actively transported by the intestinal mucosa, and variable proportions are converted to glucose in the process of absorption; in man, about one-sixth is converted ( M l ) . Most of the metabolism of fructose occurs in the liver. If a renal threshold for fructose exists, it is very low. Bickel (B4) found increased excretion of fructose in premature and newborn infants, up to 70 mg/100 ml. After the first 10 days of life, the amount of fructose was similar to that found in adults.