By Paul L. Dubin
The swift improvement of latest packings for aqueous size-exclusion chromatography has revolutionized this box. excessive solution non-adsorptive columns now make attainable the effective separation of proteins and the quick and targeted decision of the molecular weight distribution of artificial polymers. This know-how is additionally being utilized to the separation of small ions, the characterization of associating structures, and the size of branching. whilst, basic reviews are elucidating the mechanisms of a few of the chromatographicprocesses. those advancements in rules and functions are assembled for the 1st time during this ebook. primary matters are handled: the jobs of pore constitution and macromolecular dimensions, hydrophobic and electrostatic results, and the decision and keep an eye on of column potency. High-performance packings in accordance with derivatized silica are reviewed intimately. unique recommendations are completely defined, together with SEC/LALLS, inverse exclusion chromatography, and frontal area chromatography.
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54 4za 0. 34M0-47 43a 0 . 43 63a 54a 568, 258, zoa ( R a d i i i n A , evaluated a t M = 3,000 f o r p o l y e t h y l e n e g l y c o l (PEG) and a t M = 30,000 given below t h e r e s p e c t i v e r e g r e s s i o n equations). f o r Oextran, are 20 values 2 O i and 25i. We t h u s t e n t a t i v e l y conclude t h a t , a t l e a s t f o r M = 3,000, t h e r a d i u s o f g y r a t i o n o f PEG i s equal t o t h e r a d i u s o f a p r o t e i n w i t h t h e same e l u t i o n volume. A p p l y i n g t h e same a n a l y s i s t o t h e d e x t r a n data, we n o t e t h a t for this polymer, t h e "SEC r a d i u s " shows poor agreement w i t h t h e r a d i u s o f g y r a t i o n , b u t r a t h e r good agreement w i t h t h e two values o f Re.
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