Download Aqueous Size-Exclusion Chromatography by Paul L. Dubin PDF

By Paul L. Dubin

The swift improvement of latest packings for aqueous size-exclusion chromatography has revolutionized this box. excessive solution non-adsorptive columns now make attainable the effective separation of proteins and the quick and targeted decision of the molecular weight distribution of artificial polymers. This know-how is additionally being utilized to the separation of small ions, the characterization of associating structures, and the size of branching. whilst, basic reviews are elucidating the mechanisms of a few of the chromatographicprocesses. those advancements in rules and functions are assembled for the 1st time during this ebook. primary matters are handled: the jobs of pore constitution and macromolecular dimensions, hydrophobic and electrostatic results, and the decision and keep an eye on of column potency. High-performance packings in accordance with derivatized silica are reviewed intimately. unique recommendations are completely defined, together with SEC/LALLS, inverse exclusion chromatography, and frontal area chromatography.

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54 4za 0. 34M0-47 43a 0 . 43 63a 54a 568, 258, zoa ( R a d i i i n A , evaluated a t M = 3,000 f o r p o l y e t h y l e n e g l y c o l (PEG) and a t M = 30,000 given below t h e r e s p e c t i v e r e g r e s s i o n equations). f o r Oextran, are 20 values 2 O i and 25i. We t h u s t e n t a t i v e l y conclude t h a t , a t l e a s t f o r M = 3,000, t h e r a d i u s o f g y r a t i o n o f PEG i s equal t o t h e r a d i u s o f a p r o t e i n w i t h t h e same e l u t i o n volume. A p p l y i n g t h e same a n a l y s i s t o t h e d e x t r a n data, we n o t e t h a t for this polymer, t h e "SEC r a d i u s " shows poor agreement w i t h t h e r a d i u s o f g y r a t i o n , b u t r a t h e r good agreement w i t h t h e two values o f Re.

37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. J. , 16 (1967) 838-840. D. Bly, i n B. ), Gel Permeation Chromatography i n Polymer Chemistry, P h y s i c a l Methods i n Macromolecular Chemistry, Vol 2, Dekker, New York, 1972. W. J. D. Bly, Modern S i z e E x c l u s i o n Chromatography, W i l e y , New York, 1979. E. K. Oh, u n p u b l i s h e d o b s e r v a t i o n s (1984). M. Duval, B. B l o c h and S. Kohn, J. Appl. , 16 (1972) 15851602. E. Himmel, K. Oh, D. L. Chum, J. , 267 (1983) 249-265.

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