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8. Moffatt, J. G. and Khorana, H. , J. Am. Chem. Soc. 83, 663 (1961). 9. Weimann, G. and Khorana, H. , J. Am. Chem. Soc. 84^ 4329 (1962). 10. Jacob, T. M. and Khorana, H. , J. Am. Chem. Soc. 86, 1630 (1964). 11. Tener, G. , Khorana, H. , Markham, R. and Pol. E . , J. Am. Chem. Soc. 80, 6223 (1958). 12. , Drummond, G. I. and Khorana, H. , J. Am. Chem. Soc. 83, 698 (1961). 13. Agarwal, K. , Caruthers, M. , Khorana, H. , Ohtsuka, E . , RajBhandary, U. , van de Sande, J. , Weber, H. , Nature 227, 27 (1970).

83, 649 (1961). 7. Khorana, H. , Can. J. Chem. 32, 227 (1954). 8. Moffatt, J. G. and Khorana, H. , J. Am. Chem. Soc. 83, 663 (1961). 9. Weimann, G. and Khorana, H. , J. Am. Chem. Soc. 84^ 4329 (1962). 10. Jacob, T. M. and Khorana, H. , J. Am. Chem. Soc. 86, 1630 (1964). 11. Tener, G. , Khorana, H. , Markham, R. and Pol. E . , J. Am. Chem. Soc. 80, 6223 (1958). 12. , Drummond, G. I. and Khorana, H. , J. Am. Chem. Soc. 83, 698 (1961). 13. Agarwal, K. , Caruthers, M. , Khorana, H. , Ohtsuka, E . , RajBhandary, U.

15 Time FIGURE 28. Light-dependent proton translocation by recon­ stituted bacteriorhodopsin vesicles. Vesicles in A and C-F were reconstituted from delipidated bacteriorhodopsin and soybean phospholipids; the vesicles in B were made from purple membrane sheets and soybean lipids. In all assays, the total volume was 1 m l . 15 M in E and F. The protein-to-lipid ratio w a s 1:50 in A and B and 1:80 in C-F. 5 yg/ml in C and D, and 4 yg/ml in E and F. CCCP was present in D at 10 nM; c^B" was present in F at 10 yM.

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