Download Electron Paramagnetic Resonance in Biochemistry and Medicine by Rafik Galimzyanovich Sajfutdinov, Lyudmila Ivanovna Larina, PDF

By Rafik Galimzyanovich Sajfutdinov, Lyudmila Ivanovna Larina, Tamara Il'inichna Vakul'skaya, Mikhail Grigor'evich Voronkov

Electron Paramagnetic Resonance (EPR) spectroscopy - additionally occasionally termed Electron Spin Resonance spectroscopy - has manifold power makes use of in biochemistry and medication. The paramount value of EPR spectroscopy utilized to organic tissues and fluids is that it identifies the adjustments in redox techniques that give a contribution to ailment. EPR spectroscopy has come far from its unique use to notice malignant tumors. for instance, the advance and later refinement of equipment of low-temperature registration of organic tissues widened the scope of EPR spectroscopy. suggestions made attainable by way of the creation of spin labels, probes, and traps made EPR spectroscopy ever extra appropriate to biochemistry and medication, to the purpose the place in vivo reviews are being rigorously thought of. This entire publication discusses spectra of many tissues and physically fluids, and the quantitative nature of paramagnetic facilities in either general members and sufferers struggling with numerous illnesses. specific awareness is given to the EPR exam of bio-molecules, reminiscent of enzymes, polypeptides, supplements, lipids, hydrocarbons, etc., which play a necessary position in human task. This publication can be of significant curiosity to physicians focusing on many varied components. equally, biologists, biochemists, biophysicists, and chemists will locate this ebook very helpful. It has additionally been written in order that it can be used as a textbook at graduate point.

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Magn. Reson. 133:291 (1998). I. Nicholson, M. A. Foster, F. J. L. Robb, J. M. S. Hutchison and D. J. Lurie, In vivo imaging of nitroxide-free radical clearance in the rat, using radiofrequency longitudinally detected ESR imaging, J. Magn. Reson. (B), 113 :256 (1996). Yu. K. Danis, I. 1. Naktinis and L. Ch. Chernyauskene, EPR spectroscopy in internal disease clinics, Terapevticheskii arkhiv. ). N. J. F. Dodd and H. M. Swartz, The nature ofthe ESR signal in lyophilized tissue and its relevance to malignancy, Brit.

The rotational mobility of band-3 also increases monotonically with increasing the ether concentration as shown by the affinity of spin label bis(su1fo-N-succinimidyl)doxyl-2spiro-5’-azelate. ,At concentrations of up to 2 vol% ether, hemolysis of erythrocytes was negligible and the spectroscopic changes were completely reversed following its removal. Thus, the ether-induced decrease in the lipid membrane ordering near the polar head group region significantly increase the rotational mobility of band 3 and change only negligibly its state of oligomerization[25].

5 mT is readily saturated in the SHF field (Figure 2-2). A detailed analysis of the signal suggests the presence of two types ofradicals in erythrocytes. 6 mT which appears at low modulation values [8]. The intensity of the above signals was quantitatively evaluated by double-integration method: the ratio of 2. Paramagnetic Centers in the Human Biological Media 25 broad to narrow singlet areas is 6 : 1 (Figure 2-3). Unfortunately, the narrow signal defies interpreting and will not be considered further.

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