Download Molecular fluorescence: principles and applications by Bernard Valeur PDF

By Bernard Valeur

This day, fluorescence spectroscopy is a vital software of research in lots of parts. In analytical sciences, its virtue is very excessive sensitivity and selectivity - even unmarried molecules will be detected - and it achieves a excessive spatial solution and time solution together with microscopic thoughts or laser recommendations, respectively. In fabric sciences, this is often used to review constitution and dynamics of surfaces. relatively within the components of biochemistry and molecular genetics, fluorescence spectroscopy has develop into a dominating approach. including the newest imaging innovations, fluorescence spectroscopy permits a real-time commentary of the dynamics of intact organic platforms with an exceptional answer. This ebook bargains a entire creation to and survey of fluorescence spectroscopy. it really is written for newbies and energetic researchers alike who're studying to use fluorescence tools within the parts of chemistry, actual chemistry, polymers, fabrics, colloids, biochemistry, biology, scientific and pharmaceutical learn.

Show description

Read or Download Molecular fluorescence: principles and applications PDF

Similar analytic books

Principles of mass spectrometry applied to biomolecules

An in depth compilation of articles by way of top execs, this reference explains the elemental rules of mass spectrometry as they relate to the existence sciences. themes lined comprise spectroscopy, energetics and mechanisms of peptide fragmentation, electron seize dissociation, ion-ion and ion-molecule reactions, response dynamics, collisional activation, soft-landing, protein constitution and interactions, thermochemistry, and extra.

HPLC for Pharmaceutical Scientists

HPLC for Pharmaceutical Scientists is a superb ebook for either beginner and skilled pharmaceutical chemists who frequently use HPLC as an analytical instrument to resolve hard difficulties within the pharmaceutical undefined. It presents a unified method of HPLC with an equivalent and balanced remedy of the idea and perform of HPLC within the pharmaceutical undefined.

Separation Techniques in Clinical Chemistry

This reference examines ideas in separation technology for more suitable sensitivity and cost-efficiency, elevated velocity, better pattern throughput and decrease solvent intake within the review, evaluate, and validation of rising drug compounds. It investigates breakthroughs in pattern pretreatment, HPLC, mass spectrometry, capillary electrophoresis and healing drug tracking for more suitable productiveness, precision, and defense in medical chemistry, biomedical research, and forensic examine.

Importance of chirality to flavor compounds

Chiral molecules are ubiquitous in nature. hence, it's not spectacular to encounter this phenomenon on the earth of style ingredients. This e-book offers an summary at the analytical methods presently utilized to investigate chiral style elements at hint degrees. It demonstrates numerous examples for the applying of those suggestions to figure out certainly happening enantiomeric compositions of chiral key style compounds in a number of common structures.

Extra resources for Molecular fluorescence: principles and applications

Example text

12 1 Introduction Tab. 4. Information provided by fluorescent probes in various fields Field Information Polymers dynamics of polymer chains; microviscosity; free volume; orientation of chains in stretched samples; miscibility; phase separation; diffusion of species through polymer networks; end-to-end macrocyclization dynamics; monitoring of polymerization; degradation nature of the surface of colloidal silica, clays, zeolites, silica gels, porous Vycor glasses, alumina: rigidity, polarity and modification of surfaces critical micelle concentration; distribution of reactants among particles; surfactant aggregation numbers; interface properties and polarity; dynamics of surfactant solutions; partition coefficients; phase transitions; influence of additives fluidity; order parameters; lipid–protein interactions; translational diffusion; site accessibility; structural changes; membrane potentials; complexes and binding; energy-linked and lightinduced changes; effects of additives; location of proteins; lateral organization and dynamics characterization of the bilayer: microviscosity, order parameters; phase transition; effect of additives; internal pH; permeability binding sites; denaturation; site accessibility; dynamics; distances; conformational transition flexibility; torsion dynamics; helix structure; deformation due to intercalating agents; photocleavage; accessibility; carcinogenesis visualization of membranes, lipids, proteins, DNA, RNA, surface antigens, surface glycoconjugates; membrane dynamics; membrane permeability; membrane potential; intracellular pH; cytoplasmic calcium, sodium, chloride, proton concentration; redox state; enzyme activities; cell–cell and cell–virus interactions; membrane fusion; endocytosis; viability, cell cycle; cytotoxic activity fluoroimmunoassays Solid surfaces Surfactant solutions Biological membranes Vesicles Proteins Nucleic acids Living cells Fluoroimmunochemistry attached to surfactants, polymer chains, phospholipids, proteins, polynucleotides, etc.

G. in fluorescence polarization or energy transfer experiments). Environment-insensitive probes are also better suited to fluorescence microscopy and flow cytometry. A criticism often aimed at the use of extrinsic fluorescent probes is the possible local perturbation induced by the probe itself on the microenvironment to be probed. There are indeed several cases of systems perturbed by fluorescent probes. ). To minimize the perturbation, attention must be paid to the size and shape of the probe with respect to the probed region.

Concepts, Vols. 1 and 2, Marcel Dekker, New York. Cundall R. B. and Dale R. E. (Eds) (1983) Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology, Plenum Press, New York. Czarnik A. W. ) (1992) Fluorescence Chemosensors for Ion and Molecule Recognition, American Chemical Society, Washington. Demas J. N. (1983) Excited State Lifetime Measurement, Academic Press, New York. -P. and Czarnik A. W. (Eds) (1997 ) Chemosensors of Ion and Molecule Recognition, Kluwer Academic Publishers, Dordrecht.

Download PDF sample

Rated 4.25 of 5 – based on 15 votes