By Bernard Valeur
This day, fluorescence spectroscopy is a vital software of research in lots of parts. In analytical sciences, its virtue is very excessive sensitivity and selectivity - even unmarried molecules will be detected - and it achieves a excessive spatial solution and time solution together with microscopic thoughts or laser recommendations, respectively. In fabric sciences, this is often used to review constitution and dynamics of surfaces. relatively within the components of biochemistry and molecular genetics, fluorescence spectroscopy has develop into a dominating approach. including the newest imaging innovations, fluorescence spectroscopy permits a real-time commentary of the dynamics of intact organic platforms with an exceptional answer. This ebook bargains a entire creation to and survey of fluorescence spectroscopy. it really is written for newbies and energetic researchers alike who're studying to use fluorescence tools within the parts of chemistry, actual chemistry, polymers, fabrics, colloids, biochemistry, biology, scientific and pharmaceutical learn.
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Extra resources for Molecular fluorescence: principles and applications
12 1 Introduction Tab. 4. Information provided by fluorescent probes in various fields Field Information Polymers dynamics of polymer chains; microviscosity; free volume; orientation of chains in stretched samples; miscibility; phase separation; diﬀusion of species through polymer networks; end-to-end macrocyclization dynamics; monitoring of polymerization; degradation nature of the surface of colloidal silica, clays, zeolites, silica gels, porous Vycor glasses, alumina: rigidity, polarity and modiﬁcation of surfaces critical micelle concentration; distribution of reactants among particles; surfactant aggregation numbers; interface properties and polarity; dynamics of surfactant solutions; partition coeﬃcients; phase transitions; inﬂuence of additives ﬂuidity; order parameters; lipid–protein interactions; translational diﬀusion; site accessibility; structural changes; membrane potentials; complexes and binding; energy-linked and lightinduced changes; eﬀects of additives; location of proteins; lateral organization and dynamics characterization of the bilayer: microviscosity, order parameters; phase transition; eﬀect of additives; internal pH; permeability binding sites; denaturation; site accessibility; dynamics; distances; conformational transition ﬂexibility; torsion dynamics; helix structure; deformation due to intercalating agents; photocleavage; accessibility; carcinogenesis visualization of membranes, lipids, proteins, DNA, RNA, surface antigens, surface glycoconjugates; membrane dynamics; membrane permeability; membrane potential; intracellular pH; cytoplasmic calcium, sodium, chloride, proton concentration; redox state; enzyme activities; cell–cell and cell–virus interactions; membrane fusion; endocytosis; viability, cell cycle; cytotoxic activity ﬂuoroimmunoassays Solid surfaces Surfactant solutions Biological membranes Vesicles Proteins Nucleic acids Living cells Fluoroimmunochemistry attached to surfactants, polymer chains, phospholipids, proteins, polynucleotides, etc.
G. in ﬂuorescence polarization or energy transfer experiments). Environment-insensitive probes are also better suited to ﬂuorescence microscopy and ﬂow cytometry. A criticism often aimed at the use of extrinsic ﬂuorescent probes is the possible local perturbation induced by the probe itself on the microenvironment to be probed. There are indeed several cases of systems perturbed by ﬂuorescent probes. ). To minimize the perturbation, attention must be paid to the size and shape of the probe with respect to the probed region.
Concepts, Vols. 1 and 2, Marcel Dekker, New York. Cundall R. B. and Dale R. E. (Eds) (1983) Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology, Plenum Press, New York. Czarnik A. W. ) (1992) Fluorescence Chemosensors for Ion and Molecule Recognition, American Chemical Society, Washington. Demas J. N. (1983) Excited State Lifetime Measurement, Academic Press, New York. -P. and Czarnik A. W. (Eds) (1997 ) Chemosensors of Ion and Molecule Recognition, Kluwer Academic Publishers, Dordrecht.