By Professor Dr. Hildebert Wagner, Dr. Sabine Bladt, Eva Maria Zgainski (auth.)
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Additional resources for Plant Drug Analysis: A Thin Layer Chromatography Atlas
20111 are used for TLC. For Stramonii folium: 5 ml atropine sulphate soln. , and diluted to 10 ml with methanol. 20111 are used for TLC. 5. Adsorbent Silica gel 60F 2S4 pre-coated TLC plates (Merck, Darmstadt). Most of the alkaloids can be separated on silicic acid. Aluminium oxide (see Fig. 16B, p. 80) is more suitable for the separation ofberberine, columbamine and jateorhizin. 53 6. Chromatography solvents Solvent system Drug; Alkaloids AL-l Toluene-ethyl acetate-diethylamine (70: 20: 10) Screening system, suitable for the major alkaloids of most drugs.
303) Fig. lSA, B, C; 16C Fig. 16A, B VlS. Fig. lSA, C; 16C vis. Fig. 16A VlS. Fig. lSB; 16B For description of drugs see p. 12. Formulae p. 21. Chromatogram 15 A 1 Menthae piperitae aeth. After treatment with VS-reagent, the TLC of official peppermint oil is characterized by the following terpenes: No. 75 menthol piperitone cineole not identified isomenthone menthone menthyl acetate blue orange bIue blue blue-green blue-green blue III IV V VI VII 15B,16B 16A After treatment with PMA-reagent, all zones are blue-black on a yellow-green background.
303) Vanillin-sulphuric acid-reag. (VS No. 38, p. 304) Anisaldehyde-sulphuric acid-reag. (AS No. 2, p. 299) Vanillin-hydrochloric acid-reag. (VHA No. 37, p. 304) vis. Fig. 25 A vis. Fig. 25B, C; 26B VlS. 26A vis. 26C For description of drugs see p. 15-16. Formulae p. 19. Chromatogram 25 A, B 1-4 Terebinthinae aeth. Rectified turpentine oil (1) is characterized by a high content of THC (a-, ß-pinene, a-, ß-phellandrene, limonene). With PMA- or VS-reagent, these appear as blue or blue-violet zones, respectively, at the solvent front.