Download Advances in Lipid Methodology, Volume 3 by W. W. Christie PDF

By W. W. Christie

This is often the 3rd quantity of an occasional sequence of books facing elements of lipid methodology.  The members talk about and learn positional isomers of glycerolipids, long-chain acyl-coenzyme A esters, 31P nuclear magnetic resonance profiling of phospholipids and a few very important references in lipid method.

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Additional resources for Advances in Lipid Methodology, Volume 3

Example text

G. human female breast extracts, will give rise to an annoying variability in the phospholipid chemical shifts that lends a degree of uncertainty to signal identification, although such variability usually does not compromise quantification. For the purpose of signal identification, the evaporated extracted lipid sample can be treated with from 5 to 10 volumes of acetone. The phospholipids are only sparingly soluble in acetone, and by this process the acetone-insoluble phospholipids may be purged of the bulk of the neutral lipid component.

The overall correctness was assessed by comparing the reconstituted total to the total of the original triacylglycerols. In addition, the fatty acid composition of the sn-2-position of the triacylglycerols was determined directly by pancreatic lipase hydrolysis [69]. The chiral-phase HPLC method and the above calculations gave stereospecific distributions of fatty acids from synthetic and natural triacylglycerols, whi~h compared closely to those derived by enzymatic methods [30,69]. Kuksis [41] has discussed how fatty acid compositions of the various classes of the acylglycerols derived by Grignard degradation and enzymatic degradation of glycerophospholipids, prepared as intermediates in stereospecific enzymatic analyses, can be effectively cross-checked for validity.

Alternatively, the fatty acid composition of the sn-2-position has been derived from the 2-monoacylglycerol released by pancreatic lipase [16]. The results of stereospecific distribution of the fatty acids ADVANCES IN LIPID METHODOLOGY - THREE 31 determined by the separation of the diastereomeric diacylglycerols gave results, which agreed closely to those obtained previously by enzymatic methods [7], where parallel data were available. The compositions of the fatty acids in the sn-1-, sn-2- and sn- 3~positions oftriacylglycerols analysed by chiral-phase separation of the sn-1,2- and sn-2,3-diacylglycerols are also calculated indirectly [30,89,110,112,113].

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