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By Dean F. Martin and Barbara B. Martin

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2] Tswett M. , Khromofilly v Rastitel’nom i Zhivotnom Mire (Chromophylls in the Plant and Animal World). Karbasnikov Publishers, Warsaw (1910). [3] Gjerde D. T. & Fritz J. S. Ion Chromatography (2000). Weinheim: Wiley-VCH. [4] Basic principles of Ion chromatography: IC Module Navigation, The University of MACHIAS at MACHIAS. [5] Theory of Ion Chromatography, Mterohm, UK, LTD. com [7] Ion exchange chromatography: Principles and Methods, Amersham Bioscience. [8] Chromatography: a laboratory handbook of chromatographic and electrophoretic techniques Heftman, E.

Lim L. W & Takeuchi T. Determination of common inorganic anions and cations by non-suppressed ion chromatography with column switching. Journal of Chromatography A 2008; 1182 (2) 169-175. , Trefry J. H & Keaffaber J. J. Ozonation products of bromine and chlorine in seawater aquaria. Water Research 1994; 28 (5) 1087-1094. , Shellie R. A, Haddad P. R & Dicinoski G. W. Determina‐ tion of bromate in sea water using multi-dimensional matrix-elimination ion chroma‐ tography. Journal of Chromatography A 2011; 1218(50) 9080-9085.

The separation is based on the formation of ionic bonds between the charged groups of biomolecules and an ion-exchange gel/support carrying the opposite charge [7]. Biomolecules display different degrees of interaction with charged chromatography media due to their varying charge properties [8]. The earliest report of ion-exchange chromatography date back to 1850, Thompson studied the adsorption of ammonium ions to soils [9-11]. Spedding and Powell published a series of papers describing practical methods for preparative separation of the rare earths by displacement ionexchange chromatography in 1947.

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